PCR Primer Design Tool
Design PCR primers from DNA sequences and calculate Tm, GC content, and primer properties. This tool allows interactive primer selection directly on double-stranded DNA.
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What is a PCR primer?
A PCR primer is a short DNA sequence that initiates DNA synthesis during polymerase chain reaction (PCR). Proper primer design is essential for specificity and amplification efficiency.
How to design PCR primers
PCR primers are designed based on sequence length, GC content, and melting temperature (Tm). This tool allows you to visually select primer regions and automatically calculate key parameters.
Features of this tool
This PCR primer design tool enables interactive primer selection on double-stranded DNA and provides real-time evaluation of Tm, GC content, hairpin formation, and dimerization.
FAQ
What is a PCR primer?
A PCR primer is a short DNA sequence used to initiate DNA replication in PCR.
What is a good GC content for PCR primers?
A GC content of 40–60% is generally recommended.
How is melting temperature (Tm) calculated?
A simple method is Tm = 2×(A+T) + 4×(G+C), which is used in this tool.
How long should PCR primers be?
PCR primers are typically 18–25 bases long.
What causes primer-dimers?
Primer-dimers occur when primers bind to each other instead of the target DNA.
