About This Tool

This Simple PCR Primer Designer is a tool for easily designing PCR primers by selecting regions directly on a double-stranded DNA sequence. Users can move the red (forward) and blue (reverse) boxes to define their primers precisely.

The designed primers are automatically displayed on the right panel, with detailed information including:
- Sequence
- GC Content (%)
- Melting Temperature (Tm) calculated by the simple GC% method (Tm = 2×(A+T) + 4×(G+C))
- 3'-end GC presence
- Hairpin structure prediction
- Self-dimer formation prediction

All displayed primer sequences can be directly copied for immediate experimental use.

You can also highlight specific sequences by typing them into the "Annotation sequence" input and clicking "Annotate". Clicking "Clear" will remove all highlights.
This tool is optimized for quick primer design workflows, especially for basic PCR and cloning applications.